Posidonia oceanica (L.) Delile Ethanolic Extract Modulates Cell Activities with Skin Health Applications
نویسندگان
چکیده
Seagrasses are high plants sharing adaptive metabolic features with both terrestrial plants and marine algae, resulting in a phytocomplex possibly endowed with interesting biological properties. The aim of this study is to evaluate the in vitro activities on skin cells of an ethanolic extract obtained from the leaves of Posidonia oceanica (L.) Delile, family Potamogetonaceae, herein named Posidonia ethanolic extract (PEE). PEE showed high radical scavenging activity, high phenolic content, and resulted rich in chicoric acid, as determined through HPLC-MS analysis. The use of MTT assay on fibroblasts showed a PEE cytotoxicity threshold (IC05) of 50 µg/mL at 48 h, while a sub-toxic dose of 20 µg/mL induced a significant increase of fibroblast growth rate after 10 days. In addition, an ELISA assay revealed that PEE doses of 5 and 10 µg/mL induced collagen production in fibroblasts. PEE induced dose-dependent mushroom tyrosinase inhibition, up to about 45% inhibition at 1000 µg/mL, while 50% reduction of melanin was observed in melanoma cells exposed to 50 µg/mL PEE. Finally, PEE lipolytic activity was assessed by measuring glycerol release from adipocytes following triglyceride degradation. In conclusion, we have collected new data about the biological activities of the phytocomplex of P. oceanica seagrass on skin cells. Our findings indicate that PEE could be profitably used in the development of products for skin aging, undesired hyperpigmentation, and cellulite.
منابع مشابه
Hydrophilic extract from Posidonia oceanica inhibits activity and expression of gelatinases and prevents HT1080 human fibrosarcoma cell line invasion
Posidonia oceanica (L.) Delile is an endemic Mediterranean sea-grass distributed in the infralittoral zones, where it forms meadows playing a recognized ecological role in the coastal marine habitat. Although its use as a traditional herbal remedy is poorly documented, recent literature reports interesting pharmacological activities as antidiabetic, antioxidant and vasoprotective. Differently f...
متن کاملExperimental in situ exposure of the seagrass Posidonia oceanica (L.) Delile to 15 trace elements.
The Mediterranean seagrass Posidonia oceanica (L.) Delile has been used for trace element (TE) biomonitoring since decades ago. However, present informations for this bioindicator are limited mainly to plant TE levels, while virtually nothing is known about their fluxes through P. oceanica meadows. We therefore contaminated seagrass bed portions in situ at two experimental TE levels with a mix ...
متن کاملFood sources of two detritivore amphipods associated with the seagrass Posidonia oceanica leaf litter
This study focused on the ingestion and assimilation of Posidonia oceanica (L.) Delile litter by Gammarella fucicola Leach and Gammarus aequicauda Martynov, two dominant detritivore amphipods of the P. oceanica leaf litter. Scanning electron microscope observations indicated that leaf litter is highly colonized by diverse diatoms, bacteria and fungi, which may constitute a potential food source...
متن کاملHPLC Determination of Chicoric Acid in Leaves of Posidonia oceanica
In this study, Posidonia oceanica (L.) Delile (Posidoniaceae), which is a widely distributed phanerogam in Aegean and Mediterranean coasts, was investigated for the phenolic compounds that have the potential to be used in pharmaceutical sciences. The leaves, separated as young and mature leaves, were extracted and analyzed by high-performance liquid chromotagraphy (HPLC). The younger leaves wer...
متن کاملExperimental evidence for N recycling in the leaves of the seagrass Posidonia oceanica
A one-year in situ experiment using 15 N as a tracer was designed to assess the N recycling in the leaves of the seagrass Posidonia oceanica (L.) Delile. P. oceanica was shown to partly recycle the internal nitrogen pool of its leaves in order to contribute to new leaf growth. The leaves sampled in June 1999 contained 20% of the quantity of 15 N found in June 1998. N recycling caused a differen...
متن کامل